This dataset contains raw sequence data collected from an eDNA metabarcoding project to detect freshwater mussel species across two sites in Georgia (Spring Creek and Flint River) and one drainage in Missouri (Big Piney River). The eDNA samples were collected from each stream using dead-end ultra filtration (D-HFUF) with eDNA extracted from filters. We used two previously published primer sets designed to amplify freshwater mussels, one that amplified the mitochondrial cytochrome c oxidase I (COI) region and one that amplified the NADH dehydrogenase subunit I region, with negative control and mock community samples included. Samples were individually indexed, pooled, and sequenced on an Illumina MiSeq high-throughput sequencer. Raw reads were processed with bioinformatic scripts to identify unique sequences and filter out low-quality reads. Reads were assigned to mussel species using the NCBI executable BLAST v2.12.0+ and the NCBI nt database.

These data represent environmental DNA detections of freshwater mussels in the Green River, KY, USA; West Virginia long-term water quality monitoring sites, USA; and the Chesapeake Bay and Potomac basin, Maryland, USA. “This research dataset has been reviewed in accordance with U.S. Environmental Protection Agency (U.S. EPA), Office of Research and Development, and approved for release. Mention of brand names or vendors does not constitute an endorsement of products or services by the U.S. EPA.”"

Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.

Data from metabarcoding assays to detect a suite of mussel species using mitochondrial DNA regions of the cytochrome c oxidase subunit I (COI) and NADH dehydrogenase subunit (ND1) genes sequences.

Data describe a mesocosm study for the detection of mucket (Ortmanniana ligamentina), fatmucket (Lampsilis siliquoidea), and giant floater (Pyganodon grandis) freshwater mussel environmental DNA (eDNA). Water samples were collected on two different days in September of 2022 from a CERC pond containing O. ligamentina, L. siliquoidea, and P. grandis then analyzed for the presence of eDNA. Three different collection methods were performed: (1) 50 milliliter samples which were centrifuged, decanted, and concentrated pellet suspended for analysis; (2) 1.0 um mixed cellulose ester (MCE) membranes were used to filter sample material; and (3), 1.2 um polyethersulfone (PES) membranes were used to filter sample material. Parameters described in this data release include qPCR results and associated quality assurance measurements from the mesocosm water samples.

This data consists of information on the specificity and sensitivity of 4 species specific qPCR assays for the detection of eDNA from 4 species of freshwater mussels, specifically Lampsilis cardium, Lampsilis siliquoidea, Pyganodon grandis, and Anodontoides ferussacianus. The data also contain the results of eDNA sampling in Niobrara River at Niobrara National Scenic River and Agate Fossil Beds National Monument for detection of these 4 species.

This data consists of information on the specificity and sensitivity of 4 species specific qPCR assays for the detection of eDNA from 4 species of freshwater mussels, specifically Lampsilis cardium, Lampsilis siliquoidea, Pyganodon grandis, and Anodontoides ferussacianus. The data also contain the results of eDNA sampling in Niobrara River at Niobrara National Scenic River and Agate Fossil Beds National Monument for detection of these 4 species.

Positive and negative dreissenid mussel DNA quantitiative PCR results from environmental DNA water samples collected in Montana, Wisconsin and Minnesota to assess if environmental DNA can extend the seasonal window for dreissenid mussel early detection.

Positive and negative dreissenid mussel DNA quantitiative PCR results from environmental DNA water samples collected in Montana, Wisconsin and Minnesota to assess if environmental DNA can extend the seasonal window for dreissenid mussel early detection.

Environmental DNA results for water samples collected at USGS streamgages in the Columbia and Colorado river basins. Water samples were collected by USGS hydrologic technicians May - September 2018 and analyzed for presence of dreissenid mussel DNA. In this dataset, we report quantitative PCR results for each streamgage locations.