Maternal high-fat diet (HFD) and obesity can increase the susceptibility of offspring to inhaled pollutants. In this study, we examined the influence of maternal HFD on metabolic responses to ozone in young Long-Evans rat offspring. F0-females began control (CD; 10%kcal from fat) or high-fat diet (HFD; 60%kcal from fat) at post-natal day (PND)-30. Rats were bred on PND-72 and allowed to give birth. Dietary regimen was maintained until PND-30 and then all offspring were switched to CD. On PND-40, female and male offspring-F1 (n=10/group) were exposed to air or 0.8-ppm ozone for 5h and within 1h serum samples were collected for neuroendocrine hormones and global metabolomic analysis. Offspring from HFD-dams had increased body fat and weight relative to CD, however, no major changes in circulating hormones were noted. Metabolomic analysis revealed significant sex, diet, and exposure-related changes in metabolites. Maternal HFD increased free fatty acids and decreased phospholipids (female>male). Also, microbiome-associated histidine and tyrosine metabolites were increased in both sexes of offspring, while 1,5-anhydroglucitol levels decreased in males. Ozone exposure increased free fatty acids in males from CD-dams but decreased in females from HFD-dams. Ozone also decreased monohydroxy fatty acids and acyl carnitines in females (HFD>CD). Moreover, it increased pyruvate along with TCA cycle intermediates in females suggesting an increase in glucose utilization. Ozone increased various amino acids, polyamines and metabolites of gut microbiota in HFD female offspring. Collectively, these data suggest that maternal HFD increases offspring susceptibility to metabolic alterations in a sex-specific manner when challenged with environmental stressors. This dataset is associated with the following publication: Snow, S., K. Broniowska, E. Karoly, A. Henriquez, P. Phillips, A. Ledbetter, M. Schladweiler, C. Miller, C. Gordon, and U. Kodavanti. Offspring Susceptibility to Metabolic Alterations Due to Maternal High Fat Diet and the Impact of Inhaled Ozone Used as a Stressor. Scientific Reports. Nature Publishing Group, London, UK, 1, (2020).

Data from Prenatal ozone exposure programs a sexually dimorphic susceptibility to high fat diet in adolescent Long-Evans rats. This dataset is associated with the following publication: Stewart, E., J. Dye, M. Schladweiler, P. Phillips, K. McDaniel, J. Richards, R. Grindstaff, W. Padgett, M. Moore, D. Jenkins-Hill, C. Gordon, U. Kodavanti, and C. Miller. Prenatal ozone exposure programs a sexually dimorphic susceptibility to high fat diet in adolescent Long-Evans rats.. FASEB JOURNAL. Federation of American Societies for Experimental Biology (FASEB), Bethesda, MD, USA, 36(12): e22664, (2022).

This data set for this manuscript is contained within one Excel file.The different tabs of the spreadsheet pertain to each figure found in the manuscript. This file was finalized on 2/1/19 to reflect any changes made to the figures in response to reviewers comments following submission to JTEH. This dataset is associated with the following publication: Snow, S., P. Phillips, A. Ledbetter, A. Johnstone, M. Schladweiler, C. Gordon, and U. Kodavanti. The influence of maternal and perinatal high-fat diet on ozone-induced pulmonary responses in offspring. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH - PART A: CURRENT ISSUES. Taylor & Francis, Inc., Philadelphia, PA, USA, 82(2): 86-98, (2019).

This data set for this manuscript is contained within one Excel file.The different tabs of the spreadsheet pertain to each figure found in the manuscript. This file was finalized on 2/1/19 to reflect any changes made to the figures in response to reviewers comments following submission to JTEH. This dataset is associated with the following publication: Snow, S., K. Broniowska, E. Karoly, A. Henriquez, P. Phillips, A. Ledbetter, A. Johnstone, M. Schladweiler, C. Gordon, and U. Kodavanti. Offspring susceptibility to metabolic alterations due to maternal high-fat diet and the impact of inhaled ozone used as a stressor#. Scientific Reports. Nature Publishing Group, London, UK, 10(1): 16353, (2020).

In this study, we investigated effects of inhaled ozone exposure and high-cholesterol diet (HCD) in healthy Wistar and Wistar-derived Goto-Kakizaki (GK) rats, a non-obese model of type 2 diabetes. Male rats (4-week old) were fed normal diet or HCD for 12 weeks and then exposed to filtered air or 1.0 ppm ozone (6hrs/day) for 1 or 2 days. We examined pulmonary, vascular, hematology, and inflammatory responses after each exposure plus an 18-hr recovery period. This dataset is associated with the following publication: Snow, S., A. Henriquez, L. Thompson, C. Fisher, M.C. Schladweiler, C. Wood, and U. Kodavanti. Pulmonary and Vascular Effects of Acute Ozone Exposure in Diabetic Rats Fed an Atherogenic Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115430): 1, (2021).

In this study, we investigated effects of inhaled ozone exposure and high-cholesterol diet (HCD) in healthy Wistar and Wistar-derived Goto-Kakizaki (GK) rats, a non-obese model of type 2 diabetes. Male rats (4-week old) were fed normal diet or HCD for 12 weeks and then exposed to filtered air or 1.0 ppm ozone (6hrs/day) for 1 or 2 days. We examined pulmonary, vascular, hematology, and inflammatory responses after each exposure plus an 18-hr recovery period. This dataset is associated with the following publication: Snow, S., A. Henriquez, L. Thompson, C. Fisher, M.C. Schladweiler, C. Wood, and U. Kodavanti. Pulmonary and Vascular Effects of Acute Ozone Exposure in Diabetic Rats Fed an Atherogenic Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115430): 1, (2021).

pulmonary parameters, BALF biomarkers, body composition, motor activity data collected from rats exposed to ozone after high fructose or high fat diets. This dataset is associated with the following publication: Gordon , C., P. Phillips , A. Johnstone , T. Beasley , A. Ledbetter , M. Schladweiler , S. Snow, and U. Kodavanti. Effect of High Fructose and High Fat Diets on Pulmonary Sensitivity, Motor Activity, and Body Composition of Brown Norway Rats Exposed to Ozone. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 28(5): 203-15, (2016).

pulmonary parameters, BALF biomarkers, body composition, motor activity data collected from rats exposed to ozone after high fructose or high fat diets. This dataset is associated with the following publication: Gordon , C., P. Phillips , A. Johnstone , T. Beasley , A. Ledbetter , M. Schladweiler , S. Snow, and U. Kodavanti. Effect of High Fructose and High Fat Diets on Pulmonary Sensitivity, Motor Activity, and Body Composition of Brown Norway Rats Exposed to Ozone. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 28(5): 203-15, (2016).

In this study, using male Wistar and Wistar-derived Goto-Kakizaki (GK) rats, which exhibit a non-obese type-2 diabetes phenotype, we investigated whether two key metabolic stressors, type-2 diabetes and a high-cholesterol atherogenic diet, exacerbate ozone-induced metabolic effects. Rats were fed a normal control diet (ND) or high-cholesterol diet (HCD) for 12 weeks starting at 4 week-age and then exposed to filtered air or 1.0-ppm ozone (6h/day) for 1 or 2 days. Metabolic responses were analyzed at the end of each day and after an 18-hour recovery period following the 2-day exposure. This dataset is associated with the following publication: Snow, S., A. Henriquez, A. Fisher, B. Vallanat, J. House, M.C. Schladweiler, C. Wood, and U. Kodavanti. Peripheral Metabolic Effects of Ozone Exposure in Healthy and Diabetic Rats on Normal or High-Cholesterol Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115427): 1, (2021).

In this study, using male Wistar and Wistar-derived Goto-Kakizaki (GK) rats, which exhibit a non-obese type-2 diabetes phenotype, we investigated whether two key metabolic stressors, type-2 diabetes and a high-cholesterol atherogenic diet, exacerbate ozone-induced metabolic effects. Rats were fed a normal control diet (ND) or high-cholesterol diet (HCD) for 12 weeks starting at 4 week-age and then exposed to filtered air or 1.0-ppm ozone (6h/day) for 1 or 2 days. Metabolic responses were analyzed at the end of each day and after an 18-hour recovery period following the 2-day exposure. This dataset is associated with the following publication: Snow, S., A. Henriquez, A. Fisher, B. Vallanat, J. House, M.C. Schladweiler, C. Wood, and U. Kodavanti. Peripheral Metabolic Effects of Ozone Exposure in Healthy and Diabetic Rats on Normal or High-Cholesterol Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115427): 1, (2021).