,Ascomycete fungi in the genus Clarireedia are responsible for dollar spot, one of the most destructive and costly diseases affecting turfgrasses worldwide. Almost all grasses grown as turf are susceptible to dollar spot, including many high value grass species commonly used for golf courses. This Ag Data Commons dataset provides the genome sequences for seven isolates of Clarireedia fungi that cause dollar spot disease, including sequences of the two most widespread species, C. jacksonii and C. monteithiana. These data are freely available for research purposes.,,

,In a 2023 survey evaluating conifers with Rosellinia infections, five Pestalotiopsis-like fungal endophytes were isolated from plant samples obtained from Maine, New Hampshire, and Ohio by The Mycology & Nematology Genetic Diversity & Biology Laboratory at the United States Department of Agriculture. The two data sets provided herein contain species-specific base pair substitutions for the partial translation elongation factor 1-alpha gene (TEF). In the alignments, the novel Pestalotiopsis fungi are compared to their most closely related species. The data set can be used as a diagnostic tool to differentiate between the closely related species.,DNA was extracted from fungal samples using the E.Z.N.A HP Plant & Fungal DNA Kit (OMEGA® Bio-Tek, Norcross, GA, USA) following manufacturer’s protocol. The TEF locus was amplified, and reactions were conducted in 25 μL volumes with 12.5 μL of KAPA2G Robust Hotstart® (Kapa Biosystems, Inc., Wilmington, MA, USA), 1.25 μL of the forward and reverse primers at 10 μM, 1-1.75 μL of DNA at 10-20 ng, and 8 μL of molecular grade H2O. Amplifications were performed following the protocol described by Maharachchikumbura et al. (2014) in a C1000 Touch PCR Thermal Cycler (Bio-Rad, Hercules, CA). PCR products were analyzed through capillary electrophoresis with the QIAxcel Advanced System instrument and the QIAxcel ScreenGel software (Qiagen, Hilden, Germany). PCR products were then purified using ExoSAP-IT Cleanup (Affymetrix, Santa Clara, CA) following the manufacturer’s protocol. The BigDye™ 3.1 Terminator Cycle sequencing kit was used to sequence amplicons bi-directionally with the Applied Biosystems SeqStudio Genetic Analyzer (Thermo Fisher Scientific, Waltham, MA, USA).,Resources in this dataset:,,,

,Phytophthora rubi and P. fragariae are two closely related soil-borne oomycete plant pathogens that exhibit strong morphological and physiological similarities but are specialized to infect different hosts of economic importance, namely, raspberry and strawberry. Here, we report the draft genome sequences of these two Phytophthora species as a first step toward understanding the genomic processes underlying plant host adaptation in these pathogens.,,

,A new species of downy mildew (Oomycota, Peronosporales) is reported. This dataset contains alignments of DNA sequences (cox2 and LSU) markers, alignment of cox2 marker to be used in species identification and resulting phylogenetic trees.,Resources in this dataset:,

,Phenotypic evaluation of 37 crimson clover (Trifolium incarnatum L.) accessions from the US National Plant Germplasm System. Focus of the trial was on traits important for cover crop performance, including fall emergence, winter survival, flowering time, biomass, nitrogen (N) content in aboveground biomass, and proportion of plant N from biological nitrogen fixation (BNF). Experiments were conducted at the Beltsville Agricultural Research Center (Maryland, USA) across three growing seasons (2012-2013, 2013-2014, 2014-2015).,The field design was a randomized complete block design (RCBD) with four replications in each year, except for five accessions planted in 2015, which only had three replications due to limited seed availability. Each plot was a single row 0.6 m in length and 1.5 m between plots. Between 37 and 45 seeds were planted per plot, depending on seed availability in each year.,Fall emergence was evaluated in late October of each year by counting the total number of plants in each plot. Winter survival was determined by counting total number of plants per plot in late April divided by the total number of plants counted in the fall.,Flowering time was evaluated by recording percent flowering on a per-plot basis on a scale from 0% (no flower buds present) to 100% (all flowers dried up entire length of head). Flowering evaluations took place periodically between late April and early June. In 2013, evaluation took place on six dates: 23 Apr., 9 May, 15 May, 24 May, 30 May, and 4 June. In 2014, evaluation took place on five dates: 28 Apr., 6 May, 13 May, 19 May, and 27 May. In 2015, evaluation took place on eight dates: 25 Apr., 29 Apr., 4 May, 7 May, 11 May, 14 May, 18 May, and 21 May. Frequency of evaluations and total duration of evaluation period varied from year-to-year primarily due to the effects of year-to-year weather variation on the rate of growth and development.,Once an accession was rated at 50% or greater for flowering, biomass was collected. All plants in the plot were pulled up with roots attached. Plants were counted and the roots were clipped. All plants within a plot were placed in the same brown paper bag and dried. Dry weight was recorded and plants were ground for laboratory evaluation of nitrogen content, proportion of nitrogen from BNF, and metagenomic analysis.,The crimson clover biomass samples were separated into shoots and roots. Shoots were oven dried (60 °C) for approximately 72 h, weighed, and ground to pass a 1.0-mm screen. Tissue C and N concentrations and 15N natural abundance were determined for the shoot material of each accession using a Thermo Delta V Isotope Ratio Mass Spectrometer (Thermo Scientific, Waltham, MA) and Carlo Erba NC2500 Elemental Analyzer (Carlo Erba, Milan, Italy). Isotopic abundance data were expressed as δ15N in parts per thousand (‰), representing the abundance of plant tissue 15N relative to that of atmospheric N2.,,

Lab-reared colonies of Bombus huntii (Hunt bumble bee) were deployed in a commercial cherry orchard in the spring of 2016. A fungicide formulation containing boscalid (25.2%) and pyraclostrobin (12.8%) was applied one time at the recommended label rate. Nectar and pollen were collected daily, beginning two days before spray application and continuing for ten days following. Nectar samples were extracted with acetonitrile and cleaned up using dispersive solid-phase extraction with primary-secondary amine, C18, and magnesium sulfate. Pollen samples were extracted with ethyl acetate. Both types of samples were analyzed by GC-MS/MS for boscalid and pyraclostrobin. Fungicide concentrations in nectar varied spatially (by hive) and temporally. Individual pollen balls were visually identified as to primary and secondary plant sources. Pollen primarily from cherries contained the highest concentrations of the fungicides. There are 2 csv sets related to this data release.

,Protein predictions using Augustus web for the fungi Neonectria coccinea and N. faginata, as well as protein prediction of closely related species N. ditissima, and Corinectria fuckeliana.,,

,This data is collected from two experiments, one in 2018 and one in 2019, that left untreated or inoculated grapevines with Diplodia seriata, Neofusicoccum parvum, Phaeomoniella chlamydospora, or mock-inoculated, and then two months later inoculated with one of the three pathogens. Grapevine stem phenolic levels were measured at the time of the second inoculation on a different branch, and comparisons were made between pathogen infected plants or those left non-inoculated. Lesion sizes of the second inoculations also were compared to examine the effects on the first inoculation on these. Lesion lengths were measured in mm, and all phenolic compound levels were measured in mg/g FW amounts.,,