Dataset for the publication "Expanded High-Throughput Screening and Chemotype-Enrichment Analysis of the Phase II:e1k ToxCast Library for Human Sodium-Iodide Symporter (NIS) Inhibition". https://doi.org/10.1007/s00204-021-03006-2 Dataset1 contains data for figures. Dataset2 contains all supplemental tables data. This dataset is associated with the following publication: Wang, J., A. Richard, A. Murr, A. Buckalew, R. Lougee, M.A. Shobair, D. Hallinger, S. Laws, and T. Stoker. Expanded High-Throughput Screening and Chemotype-Enrichment Analysis of the Phase II:e1k ToxCast Library for Human Sodium-Iodide Symporter (NIS) Inhibition. Archives of Toxicology. Springer, New York, NY, USA, 95(5): 1723-1737, (2021).

Data pertaining to a NIS-expressing cell line, hNIS-HEK293T-EPA, and its screening capabilities for determining inhibitors of NIS-mediated iodide uptake. This dataset is associated with the following publication: Hallinger, D., A. Murr, A. Buckalew, S. Simmons, T. Stoker, and S. Laws. Development of a Screening Approach to Detect Thyroid Disrupting Chemicals that Inhibit the Human Sodium/Iodide Symporter (NIS). TOXICOLOGY IN VITRO. Elsevier Science Ltd, New York, NY, USA, 66-78, (2017).

Data pertaining to a NIS-expressing cell line, FRTL-5 and it's screening capabilities for confirming inhibitors of NIS-mediated iodide uptake. This dataset is associated with the following publication: Buckalew, A., J. Wang, A. Murr, C. Deisenroth, W. Stewart, T. Stoker, and S. Laws. Evaluation of potential sodium-iodide symporter (NIS) inhibitors using a secondary Fischer rat thyroid follicular cell (FRTL-5) radioactive iodide uptake (RAIU) assay. Archives of Toxicology. Springer, New York, NY, USA, 94(3): 873-885, (2020).

Abbreviations and data for manuscript Figures in the main text and supplemental. This dataset is associated with the following publication: Stoker, T., J. Want, A. Murr, J. Bailey, and A.R. Buckalew. High-Throughput Screening of ToxCast PFAS Chemical Library for Potential Inhibitors of the Human Sodium Iodide Symporter. CHEMICAL RESEARCH IN TOXICOLOGY. American Chemical Society, Washington, DC, USA, 36(3): 380-389, (2023).

Iodide uptake following a two-hour incubation with concentrations of 5-ATcompared to control iodide uptake in FRTL thyroid follicular cells. This dataset is associated with the following publication: Adams, V., M. Bazar, E. Reinke, A. Buckalew, and W. Eck. In vitro and in vivo effects of 5-Aminotetrazole (5-AT)- an energetic compound. REGULATORY TOXICOLOGY AND PHARMACOLOGY. Elsevier Science Ltd, New York, NY, USA, 111(104573): 1, (2020).

These excel spreadsheets contains the resultant data from inhibition assays with human Iodothyronine Deiodinase Types 1, 2, and 3 screened against the ToxCast Phase 1_v2, Phase 2, and e1k chemical libraries, as well as a few additional chemicals used in assay development. Over 1,800 chemicals were tested in total. It contains the list of chemicals tested and the median, minimum, and maximum inhibition for each chemical screened at 200 µM. Chemicals that gave greater than 50% inhibition were screened in concentration-response mode, and the median, min, max inhibition at each concentration for those chemicals are included. A model inhibitor (propylthiouracil or xanthohumol) was used in each plate as a positive control and the concentration-response data for those curves are also included. This dataset is associated with the following publication: Olker, J., J. Korte, J. Denny, P. Hartig, M. Cardon, C. Knutsen, P. Kent, J. Christensen, S. Degitz, and M. Hornung. Screening the ToxCast Phase 1, Phase 2, and e1k Chemical Libraries for Inhibitors of Iodothyronine Deiodinases. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 168(2): 430-442, (2019).

This excel file contains 7 tabs, each tabs contains the data for one specific figure in the paper. Description of the data and column names is also provided in each tab. This dataset is associated with the following publication: Wang, J., D. Hallinger, A. Murr, A. Buckalew, S. Simmons, S. Laws, and T. Stoker. High-Throughput Screening and Quantitative Chemical Ranking for Sodium Iodide Symporter Inhibitors in ToxCast Phase 1 Chemical Library. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 52(9): 5417-5426, (2018).

This dataset was generated to support parameterization of a larval amphibian thyroid axis computational model. Specifically, plasma iodine levels were determined not only to inform a concentration value for model parameter specification in the model, but also to support the notion that aquatic and terrestrial species should be considered substantially different with regard to circulating iodide levels. Other computational model physical parameters were informed by evaluating Xenopus laevis thyroid gland histological images for morphological features including thyroid follicular cell numbers and areas. This dataset is associated with the following publication: Haselman, J., J. Nichols, K. Mattingly, M. Hornung, and S. Degitz. A biologically based computational model for the hypothalamic-pituitary-thyroid (HPT) axis in Xenopus laevis larvae. MATHEMATICAL BIOSCIENCES. Elsevier Science Ltd, New York, NY, USA, 362: 109021, (2023).

Serum and brain thyroid hormone measurements in rat brain. This dataset is associated with the following publication: Gilbert, M., J. Ford, C. Riutta, K. OShaughnessy, and P.A. Kosian. Reducing Uncertainties in Quantitative Adverse Outcome Pathways by Analysis of Thyroid Hormone in the Neonatal Rat Brain. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 193(2): 192-203, (2023).