Diet of bonnethead shark in eastern Gulf of Mexico
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To examine variation in diet and daily ration of the bonnethead, Sphyrna tiburo (Linnaeus, 1758), sharks were collected from three areas in the eastern Gulf of Mexico: northwest Florida (29o40N, 85o13W), Anclote Key near Tampa Bay (28o10N, 82o42.5W), and Florida Bay (24o50N, 80o48W) from March through September, 1998-2000. In each area, diet was assessed by life stage (young-of-the year, juveniles, and adults) and quantified using five indices: percent by number (N), percent by weight (W), frequency of occurrence (O), index of relative importance expressed on a percent basis (IRI), and IRI based on diet category (IRIDC). Diet could not be assessed for young-of-the-year in Tampa Bay or Florida Bay owing to low sample size. Diet analysis showed an ontogenetic shift in northwest Florida. Young-of-the-year stomachs from northwest Florida (n68, 1 empty) contained a mix of seagrass and crustaceans while juvenile stomachs (n82, 0 empty) contained a mix of crabs and seagrass and adult stomachs (n39, 1 empty) contained almost exclusively crabs. Crabs made up the majority of both juvenile and adult diet in Tampa Bay (n79, 2 empty, and n88, 1 empty, respectively). Juvenile stomachs from Florida Bay (n72, 0 empty) contained seagrass and a mix of crustaceans while adult stomachs contained more shrimp and cephalopods (n82, 3 empty). Diets in northwest Florida and Tampa Bay were similar. The diet in Florida Bay was different from those in the other two areas, consisting of fewer crabs and more cephalopods and lobsters. Plant material was found in large quantities in all stomachs examined from all locations (15 IRIDC in 6 of the 7 life stage-area combinations, 30 IRIDC in 4 of the 7 combinations, and 62 IRIDC in young-of-the-year diet in northwest Florida). Using species- and area-specific inputs, a bioenergetic model was constructed to estimate daily ration. Models were constructed under two scenarios: assuming plant material was and was not part of the diet. Overall, daily ration was significantly different by sex, life stage, and region. The bioenergetic model predicted increasing daily ration with decreasing latitude and decreasing daily ration with ontogeny regardless of the inclusion or exclusion of plant material. These results provide evidence that bonnetheads continuously exposed to warmer temperatures have elevated metabolism and require additional energy consumption to maintain growth and reproduction.
Stomach content analysis of the Atlantic Sharpnose Shark from the Northeast Gulf of Mexico from 2000 to 2003 (NCEI Accession 0164788)
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Atlantic sharpnose shark Rhizoprionodon terraenovae diet is described from Crooked Island Sound, an embayment of the northeast Gulf of Mexico. Diet was assessed by life-stage and quantified using six indices: percent by number, percent by weight, frequency of occurrence, the index of relative importance (IRI), IRI expressed on a percent basis (IRI), and IRI based on prey category (IRIPC). Young-of-the-year sharks (n56) fed on a mix of teleosts (mostly clupeids, 44.6 IRIPC) and invertebrates (combined, 25.1 IRIPC), juveniles (n185) on sciaenids (40.7 IRIPC) and clupeids (37.8 IRIPC), and adults (n105) on sciaenids (71.4 IRIPC). Differences in diet by site and ontogeny were tested by comparing diet from Crooked Island Sound with published information from St. Vincent Island in Apalachicola Bay, an adjacent estuary. Stomach contents were also used to expand on published prey size-predator size information.
Using the otolith sulcus to aid in prey identification and improve estimates of prey size in diet studies of a piscivorous predator
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Diet studies are fundamental for understanding trophic connections in marine ecosystems. In the southeastern US, the common bottlenose dolphin Tursiops truncatus is the predominant marine mammal in coastal waters, but its role as a top predator has received little attention. Diet studies of piscivorous predators, like bottlenose dolphins, start with assessing prey otoliths recovered from stomachs or feces, but digestive erosion hampers species identification and underestimates fish weight (FW). To compensate, FW is often estimated from the least affected otoliths and scaled to other otoliths, which also introduces bias. The ulcus, an otolith surface feature, has a species-specific shape of its ostium and caudal extents, which is within the otolith edge for some species. We explored whether the sulcus could improve species identification and estimation of prey size using a case study of four sciaenid species targeted by fisheries and bottlenose dolphins in North Carolina. Methods were assessed first on otoliths from a reference collection (n=421) and applied to prey otoliths (n=5308) recovered from 20 stomachs of dead stranded dolphins. We demonstrated in reference collection otoliths that cauda to sulcus length (CL:SL) could discriminate between spotted seatrout (Cynoscion nebulosus) and weakfish (Cynoscion regalis) (classification accuracy=0.98). This method confirmed for the first time predation of spotted seatrout by bottlenose dolphins in North Carolina. Using predictive models developed from reference collection otoliths, we provided evidence that digestion affects otolith length more than sulcus or cauda length, making the latter better predictors. Lastly, we explored scenarios of calculating total consumed biomass across degrees of digestion. A suggested approach was for the least digested otoliths to be scaled to other otoliths iteratively from within the same stomach, month, or season as samples allow. Using the otolith sulcus helped overcome challenges of species identification and fish-size estimation, indicating their potential use in other diet studies.