Figure 1. Comparison of cell viability in response to exposure of cells to oleic acid (in ethanol), a hydroxy-metabolite, or methylated oleic acid as percent of the vehicle control response. Figure 2. Mitochondrial stress test extracellular flux using comparison of cellular oxygen consumption rate measures. Figure 3. Soluble mediator release comparison of cells exposed to oleic acid and its metabolites. Figure 4. Cellular iron-uptake following incubation with fatty acids. Supplement 2A. Lactate dehydrogenase activity release ( a measure of cell viability) is dose- and time-dependent. Supplement 2B. Oleic acid cellular association differs with vehicle utilized. This dataset is associated with the following publication: Bass, V., J. Soukup, A. Ghio, and M. Madden. Oleic Acid and Derivatives Affect Human Endothelial Mitochondrial Function Cell and Vasoactive Mediator Production. Lipids in Health and Disease. BioMed Central Ltd, London, UK, 19(1): 128, (2020).

Dataset for a study examining the cardiovascular effects from acute ozone exposure in rats fed with fish oil or olive oil diets. This dataset is associated with the following publication: Tong, H., S. Snow, M.C. Schladweiler, G. Carswell, B. Chorley, and U. Kodavanti. Fish Oil and Olive Oil-Enriched Diets Alleviate Acute Ozone-induced Cardiovascular Effects in Rats. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 409: 115296, (2020).

This is a manuscript developed by a group at University of Berlin and Danish Technical University, using previously published data from EPA. The only EPA interaction has been providing advice on how to correctly use the data. This dataset is not publicly accessible because: The data were not collected by EPA and are hosted external to the agency. It can be accessed through the following means: Contact the corresponding author Yasmine Emara at the Department of Environmental Technology, Technical University Berlin, 10623 Berlin, Germany. Email: y.emara@tu-berlin.de. Format: Not available. This dataset is associated with the following publication: Emara, Y., P. Fantke, R. Judson, X. Chang, P. Pradeep, A. Lehmann, M. Siegert, and M. Finkbeiner. Integrating endocrine-related health effects into comparative human toxicity characterization. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 762: 143874, (2021).

Oxidative stress (OS) is a contributing factor to the neuro, cardiac, and pulmonary effects caused by adverse metabolic states, such as obesity and type II diabetes, as well as inhalation of air borne toxicants, such as ozone (O3). The objective of this study is to understand diet/O3 interactions on OS parameters in young male Brown Norway rats maintained on regular (Purina 5001), high fructose (FRUC, TD.89247), or high fat (FAT, TD.06414) diet for 16 wks followed by exposure to either filtered air or 0.8 ppm O3 under an acute (1 d for 5 h) or subacute (5 h/d, 1 d/wk for four wks) paradigm. After 18 h of the last exposure, measures of ROS production (NAD(P)H:quinone oxidoreductase (NQO1) and NADH-Ubiquinone reductase (UBIQ-RD) levels), antioxidant homeostasis (total antioxidant substance (TAS) and γ-Glutamylcysteine synthetase (gGCS) activity), and oxidative damage (total aconitase activity and protein carbonyl (PC) content) were assayed in selected brain regions. Diet/O3 interaction did not have a global effect in the brain, but did show limited regional and OS parameter specific effects. HIP showed a significant interaction between FRUC diet/O3. Aconitase in CER showed a significant interaction between diet and O3. However, regional effects of either O3 or diet alone were more profound. Within the acute condition, there was a decrease in NQ01 and UBIQ-RD in STR and HIP, respectively, regardless of exposure. Also, CER and STR showed a change in TAS due to diet alone, while FC seemed to have a larger amount of TAS due to O3 alone. Diet appeared to affect gGCS negatively in all diet groups of HIP, and only in the FAT diet of STR. The CER also appear to have a decrease in PC in FRUC group and a general decrease in PC in all diets due to O3, while aconitase increased only in FRUC air exposed animals and control O3 exposed animals. Under the subacute condition, there was an increase of NQO1 activity in only the CER due to diets alone, while UBIQ-RD increased in only the FRUC group in FC and in both diet groups in HIP. TAS was decreased in FC only in the FAT group and a clear O3 effect where FAT increased the TAS and FRUC decreased in TAS. A significant interaction between diet/O3 was found in FC. The STR also showed a decrease in TAS in response to O3. Diet also increased PC formation within CER only in the FAT group, while HIP showed a decrease in PC after O3 exposure in controls. Aconitase in CER was affected both by diet in the filtered air group while O3 caused a decrease in controls and FRUC groups. O3 affected all groups within HIP and HYP. STR was most affected by FAT diet in both air and O3 groups. Diet seemed to be the driving factor in most OS measures. Overall, OS parameters measured do not suggest a consistent O3/diet interaction on oxidative damage pathways, but do give insight as to how high caloric diets could affect neuronal OS. This dataset is associated with the following publication: Valdez, J., A. Johnstone, J. Richards, J. Schmid, J. Royland, and P. Kodavanti. Interaction of Diet and Ozone Exposure on Oxidative Stress Parameters within Specific Brain Regions of Male Brown Norway Rats. International Journal of Molecular Sciences. MDPI AG, Basel, SWITZERLAND, 1-17, (2018).

Oxidative stress (OS) is a contributing factor to the neuro, cardiac, and pulmonary effects caused by adverse metabolic states, such as obesity and type II diabetes, as well as inhalation of air borne toxicants, such as ozone (O3). The objective of this study is to understand diet/O3 interactions on OS parameters in young male Brown Norway rats maintained on regular (Purina 5001), high fructose (FRUC, TD.89247), or high fat (FAT, TD.06414) diet for 16 wks followed by exposure to either filtered air or 0.8 ppm O3 under an acute (1 d for 5 h) or subacute (5 h/d, 1 d/wk for four wks) paradigm. After 18 h of the last exposure, measures of ROS production (NAD(P)H:quinone oxidoreductase (NQO1) and NADH-Ubiquinone reductase (UBIQ-RD) levels), antioxidant homeostasis (total antioxidant substance (TAS) and γ-Glutamylcysteine synthetase (gGCS) activity), and oxidative damage (total aconitase activity and protein carbonyl (PC) content) were assayed in selected brain regions. Diet/O3 interaction did not have a global effect in the brain, but did show limited regional and OS parameter specific effects. HIP showed a significant interaction between FRUC diet/O3. Aconitase in CER showed a significant interaction between diet and O3. However, regional effects of either O3 or diet alone were more profound. Within the acute condition, there was a decrease in NQ01 and UBIQ-RD in STR and HIP, respectively, regardless of exposure. Also, CER and STR showed a change in TAS due to diet alone, while FC seemed to have a larger amount of TAS due to O3 alone. Diet appeared to affect gGCS negatively in all diet groups of HIP, and only in the FAT diet of STR. The CER also appear to have a decrease in PC in FRUC group and a general decrease in PC in all diets due to O3, while aconitase increased only in FRUC air exposed animals and control O3 exposed animals. Under the subacute condition, there was an increase of NQO1 activity in only the CER due to diets alone, while UBIQ-RD increased in only the FRUC group in FC and in both diet groups in HIP. TAS was decreased in FC only in the FAT group and a clear O3 effect where FAT increased the TAS and FRUC decreased in TAS. A significant interaction between diet/O3 was found in FC. The STR also showed a decrease in TAS in response to O3. Diet also increased PC formation within CER only in the FAT group, while HIP showed a decrease in PC after O3 exposure in controls. Aconitase in CER was affected both by diet in the filtered air group while O3 caused a decrease in controls and FRUC groups. O3 affected all groups within HIP and HYP. STR was most affected by FAT diet in both air and O3 groups. Diet seemed to be the driving factor in most OS measures. Overall, OS parameters measured do not suggest a consistent O3/diet interaction on oxidative damage pathways, but do give insight as to how high caloric diets could affect neuronal OS. This dataset is associated with the following publication: Valdez, J., A. Johnstone, J. Richards, J. Schmid, J. Royland, and P. Kodavanti. Interaction of Diet and Ozone Exposure on Oxidative Stress Parameters within Specific Brain Regions of Male Brown Norway Rats. International Journal of Molecular Sciences. MDPI AG, Basel, SWITZERLAND, 1-17, (2018).

Effects of exposure to fatty acids on the contraction and relaxation of aortic tissue (Figure 1 A-E) and the viability of the aortic tissue (Table 1). This dataset is associated with the following publication: Bass, V., S. Snow, J. Soukup, M. Schladweiler, A. Ghio, U. Kodavanti, and M. Madden. 12-Hydroxy Oleic Acid Impairs Endothelium Dependent Vasorelaxation. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH - PART A: CURRENT ISSUES. Taylor & Francis, Inc., Philadelphia, PA, USA, 82(5): 383-386, (2020).

Effects of exposure to fatty acids on the contraction and relaxation of aortic tissue (Figure 1 A-E) and the viability of the aortic tissue (Table 1). This dataset is associated with the following publication: Bass, V., S. Snow, J. Soukup, M. Schladweiler, A. Ghio, U. Kodavanti, and M. Madden. 12-Hydroxy Oleic Acid Impairs Endothelium Dependent Vasorelaxation. JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH - PART A: CURRENT ISSUES. Taylor & Francis, Inc., Philadelphia, PA, USA, 82(5): 383-386, (2020).

This study is a randomized trial to investigate whether dietary supplementation with fish oil or olive oil modulate respiratory and cardiovascular effects of acute exposure to ozone in a controlled exposure chamber in young healthy adults. The dataset contains all the data for the outcome variables. This dataset is associated with the following publication: Chen, H., H. Tong, W. Shen, T. Montilla, M. Case, M. Almond , H. Wells, N. Alexis , D. Peden , A. Rappold, D. Diazsanchez, R. Devlin, P. Bromberg , and J. Samet. Fish oil blunts lung function decrements induced by acute exposure to ozone in young healthy adults: A randomized trial. ENVIRONMENT INTERNATIONAL. Elsevier B.V., Amsterdam, NETHERLANDS, 167(107407): 1, (2022).

This study is a randomized trial to investigate whether dietary supplementation with fish oil or olive oil modulate respiratory and cardiovascular effects of acute exposure to ozone in a controlled exposure chamber in young healthy adults. The dataset contains all the data for the outcome variables. This dataset is associated with the following publication: Chen, H., H. Tong, W. Shen, T. Montilla, M. Case, M. Almond , H. Wells, N. Alexis , D. Peden , A. Rappold, D. Diazsanchez, R. Devlin, P. Bromberg , and J. Samet. Fish oil blunts lung function decrements induced by acute exposure to ozone in young healthy adults: A randomized trial. ENVIRONMENT INTERNATIONAL. Elsevier B.V., Amsterdam, NETHERLANDS, 167(107407): 1, (2022).

The dataset contains data of air pollution, blood lipids, vascular injury markers, coagulation markers, and heart rate variability and repolarization markers. This dataset is associated with the following publication: Chen, H., S. Zhang, W. Shen, C. Salazar, A. Schneider, A. Rappold, D. Diazsanchez, R. Devlin, J. Samet, and H. Tong. Omega-3 Fatty Acids Attenuate Cardiovascular Effects of Short-term Exposure to Ambient Air Pollution. Particle and Fibre Toxicology. BioMed Central Ltd, London, UK, 19(12): 1, (2022).