,Amino acid, sugar, phenolic, and terpenoid compound level data collected from different citrus species that were healthy or infected with different CTV strains, with CTV titer and symptoms provided. This data was analyzed in a peer-reviewed manuscript, and provided for transparency or to use to evaluate variables not discussed within the manuscript (e.g. individual CTV strain effects on chemistry versus group effects).,,

,This data is collected from two experiments, one in 2018 and one in 2019, that left untreated or inoculated grapevines with Diplodia seriata, Neofusicoccum parvum, Phaeomoniella chlamydospora, or mock-inoculated, and then two months later inoculated with one of the three pathogens. Grapevine stem phenolic levels were measured at the time of the second inoculation on a different branch, and comparisons were made between pathogen infected plants or those left non-inoculated. Lesion sizes of the second inoculations also were compared to examine the effects on the first inoculation on these. Lesion lengths were measured in mm, and all phenolic compound levels were measured in mg/g FW amounts.,,

Deformed wing virus (DWV) is a major pathogen of concern to apiculture, and recent reports have indicated the local predominance and potential virulence of recombinants between DWV and a related virus, Varroa destructor virus 1 (VDV). However, little is known about the frequency and titer of VDV and recombinants relative to DWV generally. In this study, I assessed the relative occurrence and titer of DWV and VDV in public RNA-seq accessions of honey bee using a rapid, kmer-based approach. Three recombinant types were detectable graphically and corroborated by de novo assembly. Recombination breakpoints did not disrupt the capsid-encoding region, consistent with previous reports, and both VDV- and DWV-derived capsids were observed in recombinant backgrounds. High abundance of VDV kmers was largely restricted to recombinant forms. Non-metric multidimensional scaling identified genotypic clusters among DWV isolates, which was corroborated by read mapping and consensus generation. The recently described DWV-C lineage was not detected in the searched accessions. The data further highlight the utility of high-throughput sequencing to monitor viral polymorphisms and statistically test biological predictors of titer, and point to the need for consistent methodologies and sampling schemes.

,In 2017, cotton (Gossypium hirsutum L.) leafroll dwarf virus (CLRDV) was first reported in the United States. One CLRDV inoculum source includes the previous year’s cotton stalks, hence destroying cotton stalks could be effective for CLRDV management. However, tillage intensive stalk destruction methods (SDMs) can degrade southeastern soils, but a cover crop may provide short-term benefits and reduce CLRDV incidence. Therefore, we examined three SDMs (Tillage, Pull, Mow) across two cover crop levels [no cover and rye (Secale cereale L.) /clover (Trifolium incarnatum L.) mixture] and two cotton varieties to determine how cotton growth, soil penetration resistance (PR), and two CLRDV incidence sample times (pre-harvest and post-harvest) were affected across six environments during the 2021 and 2022 growing seasons. None of the SDMs affected any factors examined in this experiment, except soil PR and cotton yield. The Pull and Mow SDMs both increased soil PR compared to the Tillage SDM. An 8% yield increase (Pull > Mow) was observed, but the Tillage SDM yield did not differ from Pull or Mow SDMs. The rye/clover mixture also increased soil PR. Although cotton stands were 15% greater with no cover crop, subsequent cotton yield and fiber quality were minimally affected by cover crops. The rye/clover mixture increased post-harvest CLRDV incidence, and cotton yields were equal between cover crops. Pre-harvest CLRDV incidence probability was 0.23, but post-harvest CLRDV incidence probability was 0.71. Continuing to identify and evaluate cultural practices that reduce CLRDV incidence is imperative to prevent negative impacts.,This dataset contains all data and code required to reproduce the analyses, tables, and figures in the associated manuscript. A list of R packages used to create the aforementioned items can be found in the associated manuscript.,

지표성분(2차 대사산물), 활성화 물질 최종 분석 학습 데이터

지표성분(2차 대사산물), 활성화 물질 1차 분석 데이터

콩 모자이크바이러스 병징사진모자이크 바이러스(MV)는 두과작물(콩)에서 주로 발병하는 대표적인 병해로 작물 수확량을 감소시키는 원인임.

Rabbit hemorrhagic disease, a notifiable foreign animal disease in the USA, was reported for the first time in wild native North American rabbits and hares in April 2020 in the southwestern USA. Affected species included the Desert Cottontail (Sylvilagus audubonii), Mountain Cottontail (S. nutallii), Black-tailed Jackrabbit (Lepus californicus), and Antelope Jackrabbit (L. alleni). Desert Cottontails (n=7) and Black-tailed Jackrabbits (n=7) collected in April and May 2020 were necropsied at the U.S. Geological Survey National Wildlife Health Center (NWHC) and tested positive for Lagovirus europaeus GI.2, also known as rabbit hemorrhagic disease virus 2 (GI.2/RHDV2/b), by real-time PCR at the U.S. Department of Agriculture’s Foreign Animal Disease Diagnostic Laboratory. Gross and microscopic lesions were similar to those reported in European rabbits (Oryctolagus cuniculus) and other hare (Lepus) species with GI.2/RHDV2/b infection and included epistaxis (12/13; 92%), massive hepatocellular dissociation (14/14; 100%) and necrosis/apoptosis (11/11; 100%), pulmonary congestion (12/12; 100%), edema (12/13; 92%), and hemorrhage (11/12; 92%), and acute renal tubular injury (3/8; 38%). As in previous reports and when compared to historical cases in the NWHC diagnostic database, massive hepatocellular dissociation and necrosis/apoptosis was the most diagnostically distinct finding in GI.2/RHDV2/b-positive rabbits and hares. Based on the apparent susceptibility of North American Sylvilagus and Lepus species to fatal GI.2/RHDV2/b infection, additional work is needed to understand the host range, pathogenicity, and potential population impacts of GI.2/RHDV2/b in North America.