,A high-quality reference genome is an essential tool for applied and basic research on arthropods. Long-read sequencing technologies may be used to generate more complete and contiguous genome assemblies than alternate technologies, however, long-read methods have historically had greater input DNA requirements and higher costs than next generation sequencing, which are barriers to their use on many samples. Here, we present a 2.3 Gb de novo genome assembly of a field-collected adult female Spotted Lanternfly (Lycorma delicatula) using a single PacBio SMRT Cell. The Spotted Lanternfly is an invasive species recently discovered in the northeastern United States, threatening to damage economically important crop plants in the region. The DNA from one individual female specimen collected in Reading, Berks County, Pennsylvania was used to make one standard, size-selected library with an average DNA fragment size of ~20 kb. The library was run on one Sequel II SMRT Cell 8M, generating a total of 132 Gb of long-read sequences, of which 82 Gb were from unique library molecules, representing approximately 38x coverage of the genome. The assembly had high contiguity (contig N50 length = 1.5 Mb), completeness, and sequence level accuracy as estimated by conserved gene set analysis (96.8% of conserved genes both complete and without frame shift errors). Further, it was possible to segregate more than half of the diploid genome into the two separate haplotypes. The assembly also recovered two microbial symbiont genomes known to be associated with L. delicatula, each microbial genome being assembled into a single contig. We demonstrate that field-collected arthropods can be used for the rapid generation of high-quality genome assemblies, an attractive approach for projects on emerging invasive species, disease vectors, or conservation efforts of endangered species.,Supporting files for the manuscript "A High-Quality Genome Assembly from a Single, Field-collected Spotted Lanternfly (Lycorma delicatula) using the PacBio Sequel II System", include several intermediate versions of the assembly (raw output from Falcon, raw output from Falcon unzip, etc.) as well as the final assembly primary contigs and haplotigs (for the regions of the genome that were phased).,,

,The olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), is a specialist of fruits of the genus Olea and is a major pest of commercial olives due to their adverse impacts to olive production. In support of genomic and physiological research of the olive fly, we sequenced, assembled, and annotated two independent genomes, one from a wild-collected male and one from a wild-collected female. The resulting genomes are highly contiguous, collinear, and complete, attesting to the accuracy and quality of both assemblies. In addition to the autosomes captured as single contigs, the X and Y chromosomes were also captured as evidenced by the X chromosome showing diploid coverage in the female assembly compared to haploid coverage in the male assembly and the Y chromosome being entirely absent from the female assembly. In addition, a complete genome assembly of a known obligate symbiont to the olive fly, Candidatus Erwinia dacicola (Ca. E. dacicola), was fully captured.,Dataset includes scripts used in the assembly of the Bactrocera oleae genomes and the obligate symbiont Candidatus Erwinia dacicola genome. It can be used to replicate the steps necessary to assemble the male and female genomes described in National Center for Biotechnology Information (NCBI) BioProject: PRJNA1089778 and PRJNA1275571, male and female, respectively. It can also be used to replicate the steps to assemble the symbiont genome described in National Center for Biotechnology Information (NCBI) BioProject: PRJNA1090968. The olive fruit fly genome assembly workflow was performed on the United States Department of Agriculture (USDA) - SCINet/Mississippi State University High Performance Computing Cluster (HPC) Atlas and installed via Conda. The Ca. E. dacicola genome assembly workflow was performed on the United States Department of Agriculture (USDA) – SCINet (HPC) Ceres and installed via Conda.,

,Data include microbial count data (CFUs), 16S-rRNA copy number data (qPCR), and microbial community (microbiome) data from the guts of the invasive tephritid fruit flies, melon fly (Zeugodacus cucurbitae) and medfly (Ceratitis capitata).,Resources in this dataset:,

국립생태원에서 수행한 백두대간정밀조사 지의류 분야에서 조사되어 구축된 정보입니다. 지의류의 위치정보, 한글명, 학명이 포함되어 있습니다.

DNA sequence data for several genetic loci. This dataset is not publicly accessible because: It's already publicly available on GenBank. It can be accessed through the following means: GenBank/NCBI (http://www.ncbi.nlm.nih.gov/). Accession numbers KX890490-KX891168. Format: This dataset is DNA sequence data. It is available in GenBank. Accession numbers KX890490-KX891168. This dataset is associated with the following publication: Ware, J., E. Pilgrim, M. May, N. Donnelly, and K. Tennessen. Phylogenetic relationships of North American Gomphidae and their close relatives. Systematic Entomology. John Wiley & Sons, Inc., Hoboken, NJ, USA, 42(2): 347-358, (2017).

,The data in this repository is to provide the scripts used in the assembly of the Anastrepha ludens genome. It can be used to replicate the steps necessary to assemble the genome described in National Center for Biotechnology Information (NCBI) BioProject: PRJNA803324. This genome assembly workflow was performed on the United States Department of Agriculture (USDA) - SCINet/Mississippi State University High Performance Computing Cluster (HPC) Atlas and installed via Conda.,,

,The Homalodisca vitripennis genome was recently sequenced and annotated as part of the i5k pilot project by the Baylor College of Medicine.,The Glassy-winged sharpshooter, GWSS, (Homalodisca vitripennis) [Hemiptera: Cicadellidae], occurs naturally within the southern United States. Once restricted to the southeastern states, it was accidentally spread across the south into California. The GWSS is a voracious feeder, and can fly long distances, preferring to feed upon cultivated crops, ie. Grapevine, fruit trees, and in the nymphal stages many weeds and grasses. The GWSS is a serious threat to the viticulture industry as the primary vector of the plant-infecting bacterium, Xylella fastidiosa, Xf. The GWSS feeds on a diverse number of plants, during which the bacteria can infect many tree fruit, nut, vine, and woody ornamental crops. Glassy-winged Sharpshooter adults are ½ inch (13mm) long being fairly large for the Sharpshooter leafhopper family of insects. Sharpshooters use an ovipositor to lay eggs inside of the underside of leaves. The Sharpshooter will lay its eggs on almost any plant including cactus. The egg masses are usually composed of 10-20 eggs, but can lay more or as few as 1. Most of the egg masses have a waxy coating of brocosomes around the eggs for protection. The nymphs (5 instars) do not have wings, but develop wing pads in the 5th instar and are generally smaller than the adults, ranging in size from .07 inches (2 mm) to nearly ½ inch (13mm) long. The nymphs have very distinct red eyes. The Sharpshooter can consume about 300 times its own weight in fluids from the xylem vessels of the plants upon which it feeds, thus producing copious amounts of excreta fluid.,This dataset presents the Homalodisca vitripennis genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource (https://www.ncbi.nlm.nih.gov/assembly/GCA_000696855.1). Assembly method details will be available in a forthcoming publication.,NOTE: This gene set is an unstable pre-release (v0.5.3), and was provided to facilitate manual curation and analyses before the official gene set is released. Gene identifiers from this gene set will likely not be maintained.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,

The Nucleotide database is a collection of sequences from several sources, including GenBank, RefSeq, TPA and PDB. Genome, gene and transcript sequence data provide the foundation for biomedical research and discovery.

,Species- and sex-related differences of four laboratory tephritid fruit fly (Diptera: Tephritidae) gut microbiomes evaluated with full-length 16S rRNA PacBio Kinnex sequencing. Insect gut microbiomes are shaped by multiple endogenous and environmental factors. Our study evaluated the impacts of how host sex and species influence the microbiome in laboratory-reared tephritids fruit flies when controlled for location, time, and adult diet. We evaluated the gut microbiome of four lines of pest tephritid fruit fly adults (Bactrocera dorsalis, Bactrocera latifrons, Ceratitis capitata, Zeugodacus cucurbitae) using near full-length 16S rRNA sequencing with a PacBio Kinnex concatenation-based approach. We analyzed groups of males and females from each species at the same set of time, across four timepoints in a core insectary. Results demonstrate a clear impact of fruit fly species on the gut microbiome composition of the different fruit flies. Furthermore, for B. dorsalis, B. latifrons, and C. capitata, we saw an influence of sex on amplicon sequence variant (ASV) composition. However, while there was a separation of samples between the sexes for each timepoint, there was no characteristic male or female microbiome in all cases.,Dataset includes ASV count, taxonomy, fasta files, metadata, and R scripts. Raw sequence data has been deposited to the National Center for Biotechnology Information Sequence Read Archive (NCBI SRA) under the accession number PRJNA1196954.,

,The Baylor College of Medicine recently sequenced and annotated the Homalodisca vitripennis genome as part of the i5k pilot project.,The Glassy-winged sharpshooter, GWSS, (Homalodisca vitripennis) [Hemiptera: Cicadellidae], occurs naturally within the southern United States. Once restricted to the southeastern states, it was accidentally spread across the south into California. The GWSS is a voracious feeder, and can fly long distances, preferring to feed upon cultivated crops, ie. Grapevine, fruit trees, and in the nymphal stages many weeds and grasses. The GWSS is a serious threat to the viticulture industry as the primary vector of the plant-infecting bacterium, Xylella fastidiosa, Xf. The GWSS feeds on a diverse number of plants, during which the bacteria can infect many tree fruit, nut, vine, and woody ornamental crops. Glassy-winged Sharpshooter adults are ½ inch (13mm) long being fairly large for the Sharpshooter leafhopper family of insects. Sharpshooters use an ovipositor to lay eggs inside of the underside of leaves. The Sharpshooter will lay its eggs on almost any plant including cactus. The egg masses are usually composed of 10-20 eggs, but can lay more or as few as 1. Most of the egg masses have a waxy coating of brocosomes around the eggs for protection. The nymphs (5 instars) do not have wings, but develop wing pads in the 5th instar and are generally smaller than the adults, ranging in size from .07 inches (2 mm) to nearly ½ inch (13mm) long. The nymphs have very distinct red eyes. The Sharpshooter can consume about 300 times its own weight in fluids from the xylem vessels of the plants upon which it feeds, thus producing copious amounts of excreta fluid.,This dataset presents the Homalodisca vitripennis genome v1.0. This assembly version is the pre-release version, prior to filtering and quality control by the National Center for Biotechnology Information's GenBank resource (https://www.ncbi.nlm.nih.gov/assembly/GCA_000696855.1). Assembly method details will be available in a forthcoming publication.,If you wish to use this dataset, please follow the Baylor College of Medicine's conditions for data use: https://www.hgsc.bcm.edu/bcm-hgsc-conditions-use,