This dataset contains data from the LAMARCK controlled exposure study including DNA methylation assessments done before and 24 hours after each exposure, subclinical health outcomes measures, exposure details, and demographic information on the individual participants in the study. This dataset is not publicly accessible because: EPA cannot release personally identifiable information regarding living individuals, according to the Privacy Act and the Freedom of Information Act (FOIA). This dataset contains information about human research subjects. Because there is potential to identify individual participants and disclose personal information, either alone or in combination with other datasets, individual level data are not appropriate to post for public access. Restricted access may be granted to authorized persons by contacting the party listed. It can be accessed through the following means: The dataset can be accessed by contacting Dr. Cavin Ward-Caviness (ward-caviness.cavin@epa.gov). Format: The data is tabular data containing information on DNA methylation assessment, lung function, inflammation, controlled exposure conditions, and demographics of the LAMARCK participants. DNA methylation age has also been calculated based on the DNA methylation assessment data. This dataset is associated with the following publication: Weston, W., M. Bind, W. Cascio, R. Devlin, D. Diaz Sanchez, and C. Ward-Caviness. Accelerated aging and altered sub-clinical response to ozone exposure in young, healthy adults. Environmental Epigenetics. Oxford University Press, Cary, NC, USA, 10(1): dvae007, (2024).

Dataset contains a list of metabolites, their fold change after ozone exposure and a p value for that change. This dataset is not publicly accessible because: EPA cannot release personally identifiable information regarding living individuals, according to the Privacy Act and the Freedom of Information Act (FOIA). This dataset contains information about human research subjects. Because there is potential to identify individual participants and disclose personal information, either alone or in combination with other datasets, individual level data are not appropriate to post for public access. Restricted access may be granted to authorized persons by contacting the party listed. It can be accessed through the following means: This dataset can be accessed by contacting Dr. Robert Devlin (devlin.rober@epa.gov). Format: The dataset was sent to us by the company (Metabolon) that did the metabolite analysis, including the statistical analysis). It is an excel spreadsheet that contains a row for each of the metabolites that were identified, fold changes in each metabolite after air and ozone exposure (and the p value of the ozone-induced change), and the pathway and superpathway to which each metabolite belongs. This dataset is associated with the following publication: Cheng, W., K. Duncan, A. Ghio, C. Ward-Caviness, E. Karoly, D. Diaz-Sanchez, R. Conolly, and R. Devlin. Changes in metabolites present in lung-lining fluid following exposure to humans to ozone. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 163(2): 430-439, (2018).

Dataset contains a list of metabolites, their fold change after ozone exposure and a p value for that change. This dataset is not publicly accessible because: EPA cannot release personally identifiable information regarding living individuals, according to the Privacy Act and the Freedom of Information Act (FOIA). This dataset contains information about human research subjects. Because there is potential to identify individual participants and disclose personal information, either alone or in combination with other datasets, individual level data are not appropriate to post for public access. Restricted access may be granted to authorized persons by contacting the party listed. It can be accessed through the following means: This dataset can be accessed by contacting Dr. Robert Devlin (devlin.rober@epa.gov). Format: The dataset was sent to us by the company (Metabolon) that did the metabolite analysis, including the statistical analysis). It is an excel spreadsheet that contains a row for each of the metabolites that were identified, fold changes in each metabolite after air and ozone exposure (and the p value of the ozone-induced change), and the pathway and superpathway to which each metabolite belongs. This dataset is associated with the following publication: Cheng, W., K. Duncan, A. Ghio, C. Ward-Caviness, E. Karoly, D. Diaz-Sanchez, R. Conolly, and R. Devlin. Changes in metabolites present in lung-lining fluid following exposure to humans to ozone. TOXICOLOGICAL SCIENCES. Society of Toxicology, RESTON, VA, 163(2): 430-439, (2018).

This dataset contains genome-wide DNA methylation data from the Illumina EPIC array as well as information on the exposure given (clean air, diesel, or ozone) at each time point. Demographic and anthropomorphic information on the participants in the study is also included within the dataset. This dataset is not publicly accessible because: EPA cannot release personally identifiable information regarding living individuals, according to the Privacy Act and the Freedom of Information Act (FOIA). This dataset contains information about human research subjects. Because there is potential to identify individual participants and disclose personal information, either alone or in combination with other datasets, individual level data are not appropriate to post for public access. Restricted access may be granted to authorized persons by contacting the party listed. It can be accessed through the following means: This data can be accessed by contacting the data owners. Access to the data requires an approved Institutional Review Board application from an appropriate institution. Format: This dataset include blood biomarker measurements (DNA methylation; Ilumina EPIC Methylation array) along with demographic and anthropomorphic information on participants. In addition there is data on which controlled exposure was received (clean air or diesel or ozone) and at which time points. This dataset is associated with the following publications: Cardenas, A., R. Fadadu, L. Van Der Laan, C. Ward-Caviness, L. Granger, D. Diaz-Sanchez, M. Bind, and R. Devlin. Controlled Human Exposures to Diesel Exhaust: A Human Epigenome-Wide Experiment of Target Bronchial Epithelial Cells. Environmental Epigenetics. Oxford University Press, Cary, NC, USA, 7(1): dvab003, (2021). Bind, M., D. Rubin, A. Cardenas, R. Dhingra, C. Ward-Caviness, Z. Liu, J. Mirowsky, J. Schwartz, B. Coull, D. Diazsanchez, and R. Devlin. HETEROGENEOUS OZONE EFFECTS ON DNA METHYLOME OF BRONCHIAL CELLS: OBSERVED IN A CROSSOVER STUDY. Scientific Reports. Nature Publishing Group, London, UK, 10(1): 15739, (2020).

Pulmonary gene expression related to the regulation of DNA methylation following an exposure to ozone in rats. Epigenetic regulation of a pulmonary hypertensive gene, apelin, was also quantified. This dataset is associated with the following publication: Miller, C., J. Dye, M. Schladweiler, J. Richards, A. Ledbetter, E. Stewart, and U. Kodavanti. Acute inhalation of ozone induces DNA methylation of apelin in lungs of Long-Evans rats.. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 30(4): 178-186, (2018).

Pulmonary gene expression related to the regulation of DNA methylation following an exposure to ozone in rats. Epigenetic regulation of a pulmonary hypertensive gene, apelin, was also quantified. This dataset is associated with the following publication: Miller, C., J. Dye, M. Schladweiler, J. Richards, A. Ledbetter, E. Stewart, and U. Kodavanti. Acute inhalation of ozone induces DNA methylation of apelin in lungs of Long-Evans rats.. INHALATION TOXICOLOGY. Taylor & Francis, Inc., Philadelphia, PA, USA, 30(4): 178-186, (2018).

This data set provides the biological endpoints collected from individual animals that culminated into a published paper. This dataset is associated with the following publication: Snow, S., C. Gordon , V. Bass, M. Schladweiler , A. Ledbetter , K. Jarema , P. Phillips , A. Johnstone , and U. Kodavanti. Age-related differences in pulmonary effects of acute and subchronic episodic ozone exposures in Brown Norway rats. INHALATION TOXICOLOGY. Informa Healthcare USA, New York, NY, USA, 28(7): 313-23, (2016).

This data set provides the biological endpoints collected from individual animals that culminated into a published paper. This dataset is associated with the following publication: Snow, S., C. Gordon , V. Bass, M. Schladweiler , A. Ledbetter , K. Jarema , P. Phillips , A. Johnstone , and U. Kodavanti. Age-related differences in pulmonary effects of acute and subchronic episodic ozone exposures in Brown Norway rats. INHALATION TOXICOLOGY. Informa Healthcare USA, New York, NY, USA, 28(7): 313-23, (2016).

The dataset contains a list of cardiac and vascular biomarkers that were obtained on each day a participant visited the EPA Human Studies Facility. It also contains ozone concentrations on those days, which are publicly available on the EPA AirNow website. This dataset is not publicly accessible because: The dataset pertains to human research. It can be accessed through the following means: Requests to access data should be sent to Robert Devlin at devlin.robert@epa.gov. Format: The metadata are in the form of spreadsheets with health endpoints listed in columns and each human participant listed as a row. Similar spreadsheets contain exposure information in columns and participants in rows. This dataset is associated with the following publication: Mirowsky, J., M. Carraway, R. Dhingra, H. Tong, L. Neas, D. Diaz-Sanchez, W. Cascio, M. Case, J. Crooks, E. Hauser, E. Dowdy, W. Krause, and R. Devlin. Ozone exposure is associated with acute changes in inflammation, fibrinolysis, and endothelial cell function in coronary artery disease patients. ENVIRONMENTAL HEALTH. Academic Press Incorporated, Orlando, FL, USA, 16: 126, (2017).

The dataset contains a list of cardiac and vascular biomarkers that were obtained on each day a participant visited the EPA Human Studies Facility. It also contains ozone concentrations on those days, which are publicly available on the EPA AirNow website. This dataset is not publicly accessible because: The dataset pertains to human research. It can be accessed through the following means: Requests to access data should be sent to Robert Devlin at devlin.robert@epa.gov. Format: The metadata are in the form of spreadsheets with health endpoints listed in columns and each human participant listed as a row. Similar spreadsheets contain exposure information in columns and participants in rows. This dataset is associated with the following publication: Mirowsky, J., M. Carraway, R. Dhingra, H. Tong, L. Neas, D. Diaz-Sanchez, W. Cascio, M. Case, J. Crooks, E. Hauser, E. Dowdy, W. Krause, and R. Devlin. Ozone exposure is associated with acute changes in inflammation, fibrinolysis, and endothelial cell function in coronary artery disease patients. ENVIRONMENTAL HEALTH. Academic Press Incorporated, Orlando, FL, USA, 16: 126, (2017).