The data contain the raw quantitative polymerase chain reaction (qPCR) results after pre-amplification by polymerase chain reaction for all Ceracystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in Passive Environmental Samplers (PES).

The data contain the raw quantitative polymerase chain reaction (qPCR) results after pre-amplification by polymerase chain reaction for all Ceracystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in Passive Environmental Samplers (PES).

These data include bark and ambrosia beetle (Coleoptera: Curculionidae) species directly reared from ʻōhiʻa lehua (Metrosideros polymorpha) wood infected with either Ceratocystis lukuohia or Ceratocystis huliohia, the pathogens responsible for Rapid ʻŌhiʻa Death (ROD). Rearing took place on Kauaʻi between January 2020 and November 2021.

These data contain the study site locations and elevation information where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island.

This data release includes data and metadata containing (1) study site locations and elevation where bolts (tree stem sections) infected with Ceratocystis lukuohia and/or C. huliohia were collected on Hawai'i Island, (2) the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts, (3) Ceratocystis spp. viability results from culturing tests of the beetles' frass, (4) frass production estimates from three ambrosia beetle species, and (5) quantitative Polymerase Chain Reaction (qPCR) DNA results of viability subcultures. Data were used in the analysis for the manuscript "Ambrosia beetle communities and frass production in ʻōhiʻa (Metrosideros polymorpha) infected with Ceratocystis fungi responsible for Rapid ʻŌhiʻa Death." Beetles and frass were collected in the lab from Rapid 'Ōhiʻa Death-infected trees from 2018-2019.

This data release includes metadata and tabular datasets that document (1) Ceratocystis qPCR DNA detections in Passive Environmental Samplers (PES), (2) Ceratocystis qPCR DNA detections after pre-amplification, (3) wood and frass particle counts detected in PES (4) location of individual PES within the study area (5) ambrosia beetle gallery counts on felled ʻōhiʻa (6) ambrosia beetle gallery counts on insecticide treated bolts and (7) ambrosia beetles trapped in cross-vein panel traps. Data were used in the analysis of the manuscript "Effectiveness of Rapid ʻŌhiʻa Death management strategies at a focal disease outbreak on Hawaiʻi Island."

These data contain the results of ʻōhiʻa seedlings challenged with different ambrosia beetle (Coleoptera: Curculionidae) species that were exposed to either Ceratocystis lukuohia or Ceratocystis huliohia in culture. Disease development, viability, and DNA confirmation data were recorded.

These data contain the results of ʻōhiʻa seedlings challenged with different ambrosia beetle (Coleoptera: Curculionidae) species that were exposed to either Ceratocystis lukuohia or Ceratocystis huliohia in culture. Disease development, viability, and DNA confirmation data were recorded.

These data include the ambrosia beetle (Coleoptera: Scolytinae) species that were reared from bolts (tree stem sections) infected with Rapid ʻŌhiʻa Death on Hawaiʻi Island from 2018-2019. These data were used to determine species, number of individuals reared, height, and elevation in which these beetles live.

These data contain the raw quantitative polymerase chain reaction (qPCR) results for all Ceratocystis lukuohia and huliohia testing of environmental DNA (eDNA) collected in the wind column using Passive Environmental Samplers (PES).