Data from: Pyrosequencing-Based Analysis of the Microbiome Associated with the Horn Fly, Haematobia irritans
공공데이터포털
,The bacterial 16S tag-encoded FLX-titanium amplicon pyrosequencing (bTEFAP) method was used to carry out the classification analysis of bacterial flora in adult female and male horn flies and horn fly eggs.,The bTEFAP method identified 16S rDNA sequences in our samples which allowed the identification of various prokaryotic taxa associated with the life stage examined. This is the first comprehensive report of bacterial flora associated with the horn fly using a culture-independent method. Several rumen, environmental, symbiotic and pathogenic bacteria associated with the horn fly were identified and quantified. This is the first report of the presence of Wolbachia in horn flies of USA origin and is the first report of the presence of Rikenella in an obligatory blood feeding insect.,Adult horn flies were collected on a single date from pastured cattle at the Louisiana State University Agricultural Center, St. Gabriel Research Station using aerial nets. Within 1 h after collection the flies were transferred to large sterile Erlenmeyer flasks and maintained in total darkness for 1.5 h and 30°C to allow flies to oviposit on the flask bottom [73]. Adult flies were released from the flasks into a cage and eggs were collected by rinsing with distilled water onto a filter paper. Both the eggs and adult flies were frozen at −80°C. To preserve nucleic acid integrity, adults were sexed on dry ice prior to freezing. Each sample used for DNA extraction and pyrosequencing consisted of 5 adult males, 5 adult females or 50 eggs pooled together and homogenized. Three replicates of adult male, adult female and eggs were analyzed.,The horn fly, Haematobia irritans, is one of the most economically important pests of cattle. Insecticides have been a major element of horn fly management programs. Growing concerns with insecticide resistance, insecticide residues on farm products, and non-availability of new generation insecticides, are serious issues for the livestock industry. Alternative horn fly control methods offer the promise to decrease the use of insecticides and reduce the amount of insecticide residues on livestock products and give an impetus to the organic livestock farming segment. The horn fly, an obligatory blood feeder, requires the help of microflora to supply additional nutrients and metabolize the blood meal. Recent advancements in DNA sequencing methodologies enable researchers to examine the microflora diversity independent of culture methods.,,
Data from: Defensive aphid symbiont Hamiltonella defensa effects on Aphelinus glycinis and Aphelinus atriplicis
공공데이터포털
,Endosymbiont interactions with hosts have important effects on fitness, including the fitness of many pest and beneficial species. Among these interactions, facultative endosymbiotic bacteria can protect aphids from parasitoids. Aphis craccivora and Acyrthosiphon pisum can harbor the symbiotic bacteria Hamiltonella defensa and its bacteriophage APSE. Infection by H. defensa defends these aphids against some but not all parasitoid species in the hymenopteran family Braconidae. Here, we report results on the effect of H. defensa on parasitism of these aphids by species in the other major lineage of aphid parasitoids, Aphelinus species in the family Aphelinidae. Parasitism of aphids infected with H. defensa /APSE by two Aphelinus species did not differ from that of uninfected aphids. While Aphelinus atriplicis showed no difference in fitness components between infected and uninfected aphids, Aphelinus glycinis actually produced more adult progeny and larger female progeny on infected than on uninfected aphids. Aphelinus glycinis may increase host quality for itself by changing the titer of nutritional versus protective bacteria in such a way that aphids infected with H. defensa can be made more suitable for parasitoid development than uninfected aphids. Our results and reasoning suggest that these Aphelinus species may be less prone to harm by H. defensa /APSE that affect eggs because they have anhydropic, heavily chorionated eggs, which may not absorb toxins during embryogenesis.,See the included methods-DefensiveAphidSymbiont.txt file for more information on the methods and design structure of this study.,
Data from: Genome analyses of fungal pathogens Neonectria faginata and Neonectria coccinea
공공데이터포털
,Protein predictions using Augustus web for the fungi Neonectria coccinea and N. faginata, as well as protein prediction of closely related species N. ditissima, and Corinectria fuckeliana.,,
Data from: Genetic mapping and QTL analysis for peanut smut resistance
공공데이터포털
,This collection contains supplementary information for the manuscript “Genetic mapping and QTL analysis for peanut smut resistance”, which reports the genetic map and quantitative trait loci associated with resistance to peanut smut, a disease caused by the fungus Thecaphora frezii. The information includes genotyping data of a 103 recombinant inbred line (RIL) population {susceptible Arachis hypogaea subsp.hypogaea × resistant synthetic amphidiploid [(A. correntina × A. cardenasii) × A. batizocoi]⁴ˣ} and parental lines, generated with the Axiom_Arachis2 SNP array.,,For more information about this dataset contact: Renee Arias: Renee.Arias@usda.gov or Alicia Massa: Alicia.Massa@usda.gov,,
Data from: Niche partitioning and coexistence of parasitoids of the same feeding guild introduced for biological control of an invasive forest pest
공공데이터포털
,The data set is collected to evaluate if two parasitoids (Spathius galinae and Tetrastichus planipennisi), introduced for biocontrol of the invasive emerald ash borer (EAB), Agrilus planipennis, into North America have established niche-partitioning, co-existing populations following their sequential or simultaneous field releases to 12 hard-wood forests located in Midwest and Northeast regions of the United States. Ash trees of various sizes (large, pole-size and saplings) were debarked meter by meter in early spring of 2019 (Michigan sites) or fall of 2019 (Northeast states: Connecticut, Massachusetts and New York). Detailed data collection procedures can be found in the associated publication in Biological Control.,,
Data from: Whole Genome Sequences of the Raspberry and Strawberry Pathogens Phytophthora rubi and P. fragariae
공공데이터포털
,Phytophthora rubi and P. fragariae are two closely related soil-borne oomycete plant pathogens that exhibit strong morphological and physiological similarities but are specialized to infect different hosts of economic importance, namely, raspberry and strawberry. Here, we report the draft genome sequences of these two Phytophthora species as a first step toward understanding the genomic processes underlying plant host adaptation in these pathogens.,,
Buse Francisella Medium
공공데이터포털
This dataset contains colony forming unit and qPCR data. This dataset is associated with the following publication: Morris, B., H. Buse, N. Adcock, and E. Rice. A novel broth medium for enhanced growth of Francisella tularensis. Letters in Applied Microbiology. Blackwell Publishing, Malden, MA, USA, 64(6): 393-468, (2017).