Data in this dataset were collected as a part of the Student Network for Amphibian Pathogen Surveilance (SNAPs) program throughout the United States by undergraduate students in biology or ecology courses as a part of their curriculum throughout 2022. This data was collected in the field by students and sent to the USGS National Wildlife Health Center (NWHC) for testing of two amphibian fungal pathogens, Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bs). The dataset includes both the field records of the individual amphibians tested and the results for individuals for Bd and Bs.

Data in this dataset were collected as a part of the Student Network for Amphibian Pathogen Surveilance (SNAPs) program throughout the United States by undergraduate students in biology or ecology courses as a part of their curriculum throughout 2022. This data was collected in the field by students and sent to the USGS National Wildlife Health Center (NWHC) for testing of two amphibian fungal pathogens, Batrachochytrium dendrobatidis (Bd) and Batrachochytrium salamandrivorans (Bs). The dataset includes both the field records of the individual amphibians tested and the results for individuals for Bd and Bs.

This dataset provides the results of a national survey of the United States for the salamander chytrid fungus Batrachochytrium salamandrivorans (Bsal) and the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) conducted by the U.S. Geological Survey Amphibian Research and Monitoring Initiative from May 2014 to August 2017. Sites were sampled by capturing amphibians by hand or by traps or nets that were then swabbed individually using methods that prevent sample contamination. All swabs were then analyzed using a real-time TaqMan PCR for detection of B. salamandrivorans and Bd on the extracted DNA. The data consist of locality information and data on the individual sampled, as well as the result of the test for B. salamandrivorans and Bd. No cases of B. salamandrivorans were detected in this study.

This dataset provides the results of a national survey of the United States for the salamander chytrid fungus Batrachochytrium salamandrivorans (Bsal) and the amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) conducted by the U.S. Geological Survey Amphibian Research and Monitoring Initiative from May 2014 to August 2017. Sites were sampled by capturing amphibians by hand or by traps or nets that were then swabbed individually using methods that prevent sample contamination. All swabs were then analyzed using a real-time TaqMan PCR for detection of B. salamandrivorans and Bd on the extracted DNA. The data consist of locality information and data on the individual sampled, as well as the result of the test for B. salamandrivorans and Bd. No cases of B. salamandrivorans were detected in this study.

This dataset includes results from external skin swabbing of Anaxyrus williamsi and Lithobates catesbeianus to test for the fungus Batrachochytrium dendrobatidis from the Dixie Valley in Churchill County, Nevada, in 2019-2021.

This dataset includes results from external skin swabbing of Anaxyrus williamsi and Lithobates catesbeianus to test for the fungus Batrachochytrium dendrobatidis from the Dixie Valley in Churchill County, Nevada, in 2019-2021.

Results of a literature search performed December 1, 2020. The tables in this document summarize the publications fitting in this search criteria and are split into the publications that update the list of international species that could carry Bsal (Appendix 1 Table S1). Number of individual live amphibians imported in categories of urodelan (salamander, newt, and related taxa) species restricted under the interim regulations and not listed, species not listed on the regulations that have had Bsal detected, notably Bombina spp., anuran species in the genus Rana, and Xenopus (including African clawed frogs) as a comparison of species not thought to be susceptible to Bsal, but commonly imported (Appendix 3 Table S1). Data and relative risk scores per county used for the risk analysis of B. salamandrivorans invasion, consequences, and surveillance for U.S. salamanders. All variables were scaled from 1, lowest relative risk, to 4, highest relative risk. Import and introduction risk scores were calculated from the average live imports per year for the pre-action risk period (2010-2015) and post-action risk period (2016-2019) (Appendix S3 Table S2).

Results of a literature search performed December 1, 2020. The tables in this document summarize the publications fitting in this search criteria and are split into the publications that update the list of international species that could carry Bsal (Appendix 1 Table S1). Number of individual live amphibians imported in categories of urodelan (salamander, newt, and related taxa) species restricted under the interim regulations and not listed, species not listed on the regulations that have had Bsal detected, notably Bombina spp., anuran species in the genus Rana, and Xenopus (including African clawed frogs) as a comparison of species not thought to be susceptible to Bsal, but commonly imported (Appendix 3 Table S1). Data and relative risk scores per county used for the risk analysis of B. salamandrivorans invasion, consequences, and surveillance for U.S. salamanders. All variables were scaled from 1, lowest relative risk, to 4, highest relative risk. Import and introduction risk scores were calculated from the average live imports per year for the pre-action risk period (2010-2015) and post-action risk period (2016-2019) (Appendix S3 Table S2).

Data described herein was collected by USGS personnel and the project funded by USGS and the National Park Service (Catoctin Mountain Park) through a FY14 NRPP project involving both agencies and developed to detect the presence and prevalence of ranavirus and chytrid pathogens among amphibians of Catoctin Mountain Park (CATO). Supplemental molecular analyses were performed on behalf of LSC by the molecular biology laboratory at Hagerstown Community College whereby students performed the molecular diagnostic testing and provided the raw data to LSC for analysis. There is no data sharing agreement specifying ownership, but the data was presented to the NPS CATO staff to help guide their management decision-making related to amphibians and disease susceptibility. This body of data is stored in spreadsheet format.

We generated a data set from by collecting duplicate swabs from all amphibian individuals sampled at 5 sites (96 individuals) and analyzed these duplicate swabs running 3 subsamples of the extracted DNA in separate qPCR reactions. The resulting data set included 2 swabs per individual with 1 qPCR result and a 2nd swab with 3 qPCR results for detection of Batrachochytrium dendrobatidis (Bd) DNA. We generated data to assess the ideal laboratory functional relationship between quantity of target Bd DNA and detection, as well as limits of detection (LOD) and quantification (LOQ) for the assay, 5 µL of synthetic double-stranded Bd DNA template (gBlocks™ Gene Fragments, Integrated DNA Technologies, Coralville, IA) ranging from 1.46 x 10-3 fg to 3 fg (approximately 6.6 – 13,000 copies) per reaction at 2-fold dilutions were each run 80 times on 10 separate plates (12 dilution series per plate).