Decomposition data of bacterial and viral fecal indicators in common human pollution types. This dataset is associated with the following publication: Wanjugi, P., M. Sivaganesan, A. Korajkic, C. Kelty, B. McMinn, R. Ulrich, V. Harwood, and O. Shanks. Differential Decomposition of Bacterial and Viral Fecal Indicators in Common Human Pollution Types. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 105: 591-601, (2016).

Decomposition data of bacterial and viral fecal indicators in common human pollution types. This dataset is associated with the following publication: Wanjugi, P., M. Sivaganesan, A. Korajkic, C. Kelty, B. McMinn, R. Ulrich, V. Harwood, and O. Shanks. Differential Decomposition of Bacterial and Viral Fecal Indicators in Common Human Pollution Types. WATER RESEARCH. Elsevier Science Ltd, New York, NY, USA, 105: 591-601, (2016).

Concentration estimates for HF183/BFDrev and HumM2 qPCR genetic markers in raw sewage collected from 54 geographic locations across the United States. This dataset is associated with the following publication: Boehm, A., J. Soller, and O. Shanks. Human-Associated Fecal qPCR Measurements and Predicted Risk of Gastrointestinal Illness in Recreational Waters Contaminated with Raw Sewage. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 2: 270-275, (2015).

Concentration estimates for HF183/BFDrev and HumM2 qPCR genetic markers in raw sewage collected from 54 geographic locations across the United States. This dataset is associated with the following publication: Boehm, A., J. Soller, and O. Shanks. Human-Associated Fecal qPCR Measurements and Predicted Risk of Gastrointestinal Illness in Recreational Waters Contaminated with Raw Sewage. ENVIRONMENTAL SCIENCE & TECHNOLOGY. American Chemical Society, Washington, DC, USA, 2: 270-275, (2015).

Log10 fecal score ratios for eligible microbial source tracking qPCR assays. This dataset is associated with the following publication: Brumfield, K.D., J.A. Cotruvo, O. Shanks, M. Sivaganesan, J. Hey, N.A. Hasan, A. Huq, R.R. Colwell, and M.B. Leddy. Metagenomic Sequencing and Quantitative Real-Time PCR for Fecal Pollution Assessment in an Urban Watershed. Frontiers in Water. Frontiers, Lausanne, SWITZERLAND, 3: 626849, (2021).

EPA Method 1615 measures enteroviruses and noroviruses present in environmental and drinking waters. The viral ribonucleic acid (RNA) from water sample concentrates is extracted and tested for enterovirus and norovirus RNA using reverse transcription-quantitative PCR (RT-qPCR). Virus concentrations for the molecular assay are calculated in terms of genomic copies of viral RNA per liter based upon a standard curve. The method uses a number of quality controls to increase data quality and to reduce interlaboratory and intralaboratory variation. The method has been evaluated by examining virus recovery from ground and reagent grade waters seeded with poliovirus type 3 and murine norovirus as a surrogate for human noroviruses. Mean poliovirus recoveries were 20% in groundwaters and 44% in reagent grade water. Mean murine norovirus recoveries with the RT-qPCR assay were 31% in groundwaters and 4% in reagent grade water. This dataset is associated with the following publication: Fout , S., J. Cashdollar , S. Griffin , N. Brinkman , E. Varughese , and S. Parshionikar. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR. Journal of Visualized Experiments. JoVE, Somerville, MA, USA, 107: e52646, (2016).

EPA Method 1615 measures enteroviruses and noroviruses present in environmental and drinking waters. The viral ribonucleic acid (RNA) from water sample concentrates is extracted and tested for enterovirus and norovirus RNA using reverse transcription-quantitative PCR (RT-qPCR). Virus concentrations for the molecular assay are calculated in terms of genomic copies of viral RNA per liter based upon a standard curve. The method uses a number of quality controls to increase data quality and to reduce interlaboratory and intralaboratory variation. The method has been evaluated by examining virus recovery from ground and reagent grade waters seeded with poliovirus type 3 and murine norovirus as a surrogate for human noroviruses. Mean poliovirus recoveries were 20% in groundwaters and 44% in reagent grade water. Mean murine norovirus recoveries with the RT-qPCR assay were 31% in groundwaters and 4% in reagent grade water. This dataset is associated with the following publication: Fout , S., J. Cashdollar , S. Griffin , N. Brinkman , E. Varughese , and S. Parshionikar. EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR. Journal of Visualized Experiments. JoVE, Somerville, MA, USA, 107: e52646, (2016).

This study provides valuable insights into the decay rates of wastewater-associated markers and enteric viruses under different experimental conditions that mimicked temperate environmental conditions. The findings contribute to our understanding of the fate and persistence of these markers in the environment which is crucial for assessing and managing risks from contamination by untreated human wastewater. This dataset is not publicly accessible because: no primary/secondary data owned by EPA. It can be accessed through the following means: Data will be made available on request to the corresponding author, Dr Warish Ahmed. Format: N/A- no primary/secondary data owned by EPA. This dataset is associated with the following publication: Ahmed, W., A. Korajkic, W.J.M. Smith, S. Payyappat, M. Cassidy, N. Harrison, and C. Besley. Comparing the decay of human wastewater-associated markers and enteric viruses in laboratory microcosms simulating estuarine waters in a temperate climatic zone using qPCR/RT-qPCR assays. SCIENCE OF THE TOTAL ENVIRONMENT. Elsevier BV, AMSTERDAM, NETHERLANDS, 908: 167845, (2024).

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