Data from Prenatal ozone exposure programs a sexually dimorphic susceptibility to high fat diet in adolescent Long-Evans rats. This dataset is associated with the following publication: Stewart, E., J. Dye, M. Schladweiler, P. Phillips, K. McDaniel, J. Richards, R. Grindstaff, W. Padgett, M. Moore, D. Jenkins-Hill, C. Gordon, U. Kodavanti, and C. Miller. Prenatal ozone exposure programs a sexually dimorphic susceptibility to high fat diet in adolescent Long-Evans rats.. FASEB JOURNAL. Federation of American Societies for Experimental Biology (FASEB), Bethesda, MD, USA, 36(12): e22664, (2022).

Maternal high-fat diet (HFD) and obesity can increase the susceptibility of offspring to inhaled pollutants. In this study, we examined the influence of maternal HFD on metabolic responses to ozone in young Long-Evans rat offspring. F0-females began control (CD; 10%kcal from fat) or high-fat diet (HFD; 60%kcal from fat) at post-natal day (PND)-30. Rats were bred on PND-72 and allowed to give birth. Dietary regimen was maintained until PND-30 and then all offspring were switched to CD. On PND-40, female and male offspring-F1 (n=10/group) were exposed to air or 0.8-ppm ozone for 5h and within 1h serum samples were collected for neuroendocrine hormones and global metabolomic analysis. Offspring from HFD-dams had increased body fat and weight relative to CD, however, no major changes in circulating hormones were noted. Metabolomic analysis revealed significant sex, diet, and exposure-related changes in metabolites. Maternal HFD increased free fatty acids and decreased phospholipids (female>male). Also, microbiome-associated histidine and tyrosine metabolites were increased in both sexes of offspring, while 1,5-anhydroglucitol levels decreased in males. Ozone exposure increased free fatty acids in males from CD-dams but decreased in females from HFD-dams. Ozone also decreased monohydroxy fatty acids and acyl carnitines in females (HFD>CD). Moreover, it increased pyruvate along with TCA cycle intermediates in females suggesting an increase in glucose utilization. Ozone increased various amino acids, polyamines and metabolites of gut microbiota in HFD female offspring. Collectively, these data suggest that maternal HFD increases offspring susceptibility to metabolic alterations in a sex-specific manner when challenged with environmental stressors. This dataset is associated with the following publication: Snow, S., K. Broniowska, E. Karoly, A. Henriquez, P. Phillips, A. Ledbetter, M. Schladweiler, C. Miller, C. Gordon, and U. Kodavanti. Offspring Susceptibility to Metabolic Alterations Due to Maternal High Fat Diet and the Impact of Inhaled Ozone Used as a Stressor. Scientific Reports. Nature Publishing Group, London, UK, 1, (2020).

Maternal high-fat diet (HFD) and obesity can increase the susceptibility of offspring to inhaled pollutants. In this study, we examined the influence of maternal HFD on metabolic responses to ozone in young Long-Evans rat offspring. F0-females began control (CD; 10%kcal from fat) or high-fat diet (HFD; 60%kcal from fat) at post-natal day (PND)-30. Rats were bred on PND-72 and allowed to give birth. Dietary regimen was maintained until PND-30 and then all offspring were switched to CD. On PND-40, female and male offspring-F1 (n=10/group) were exposed to air or 0.8-ppm ozone for 5h and within 1h serum samples were collected for neuroendocrine hormones and global metabolomic analysis. Offspring from HFD-dams had increased body fat and weight relative to CD, however, no major changes in circulating hormones were noted. Metabolomic analysis revealed significant sex, diet, and exposure-related changes in metabolites. Maternal HFD increased free fatty acids and decreased phospholipids (female>male). Also, microbiome-associated histidine and tyrosine metabolites were increased in both sexes of offspring, while 1,5-anhydroglucitol levels decreased in males. Ozone exposure increased free fatty acids in males from CD-dams but decreased in females from HFD-dams. Ozone also decreased monohydroxy fatty acids and acyl carnitines in females (HFD>CD). Moreover, it increased pyruvate along with TCA cycle intermediates in females suggesting an increase in glucose utilization. Ozone increased various amino acids, polyamines and metabolites of gut microbiota in HFD female offspring. Collectively, these data suggest that maternal HFD increases offspring susceptibility to metabolic alterations in a sex-specific manner when challenged with environmental stressors. This dataset is associated with the following publication: Snow, S., K. Broniowska, E. Karoly, A. Henriquez, P. Phillips, A. Ledbetter, M. Schladweiler, C. Miller, C. Gordon, and U. Kodavanti. Offspring Susceptibility to Metabolic Alterations Due to Maternal High Fat Diet and the Impact of Inhaled Ozone Used as a Stressor. Scientific Reports. Nature Publishing Group, London, UK, 1, (2020).

Data relative to the paper entitled " Ozone-induced fetal growth restriction in rats is associated with sexually dimorphic placental and fetal metabolic adaptation.". This dataset is associated with the following publication: Miller, C., J. Dye, A. Henriquez, E. Stewart, K. Lavrich, G. Carswell, H. Ren, D. Freeborn, S. Snow, M.C. Schladweiler, J. Richards, P.R. Kodavanti, A. Fisher, B. Chorley, and U. Kodavanti. Ozone-induced fetal growth restriction in rats is associated with sexually dimorphic placental and fetal metabolic adaptation.. Molecular Metabolism. Elsevier B.V., Amsterdam, NETHERLANDS, 20(42): 101094, (2020).

Data relative to the paper entitled " Ozone-induced fetal growth restriction in rats is associated with sexually dimorphic placental and fetal metabolic adaptation.". This dataset is associated with the following publication: Miller, C., J. Dye, A. Henriquez, E. Stewart, K. Lavrich, G. Carswell, H. Ren, D. Freeborn, S. Snow, M.C. Schladweiler, J. Richards, P.R. Kodavanti, A. Fisher, B. Chorley, and U. Kodavanti. Ozone-induced fetal growth restriction in rats is associated with sexually dimorphic placental and fetal metabolic adaptation.. Molecular Metabolism. Elsevier B.V., Amsterdam, NETHERLANDS, 20(42): 101094, (2020).

Data related to the effects of peri-implantation ozone exposure on maternal and fetal health outcomes at the end of gestation. This dataset is associated with the following publication: Miller, C., J. Dye, A. Ledbetter, M. Schladweiler, J. Richards, S. Snow, C. Wood, A. Henriquez, L. Thompson, A. Farraj, M. Hazari, and U. Kodavanti. Uterine Artery Flow and Offspring Growth in Long-Evans Rats following Maternal Exposure to Ozone during Implantation. ENVIRONMENTAL HEALTH PERSPECTIVES. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, USA, 125(12): 127005, (2017).

Data related to the effects of peri-implantation ozone exposure on maternal and fetal health outcomes at the end of gestation. This dataset is associated with the following publication: Miller, C., J. Dye, A. Ledbetter, M. Schladweiler, J. Richards, S. Snow, C. Wood, A. Henriquez, L. Thompson, A. Farraj, M. Hazari, and U. Kodavanti. Uterine Artery Flow and Offspring Growth in Long-Evans Rats following Maternal Exposure to Ozone during Implantation. ENVIRONMENTAL HEALTH PERSPECTIVES. National Institute of Environmental Health Sciences (NIEHS), Research Triangle Park, NC, USA, 125(12): 127005, (2017).

In this study, using male Wistar and Wistar-derived Goto-Kakizaki (GK) rats, which exhibit a non-obese type-2 diabetes phenotype, we investigated whether two key metabolic stressors, type-2 diabetes and a high-cholesterol atherogenic diet, exacerbate ozone-induced metabolic effects. Rats were fed a normal control diet (ND) or high-cholesterol diet (HCD) for 12 weeks starting at 4 week-age and then exposed to filtered air or 1.0-ppm ozone (6h/day) for 1 or 2 days. Metabolic responses were analyzed at the end of each day and after an 18-hour recovery period following the 2-day exposure. This dataset is associated with the following publication: Snow, S., A. Henriquez, A. Fisher, B. Vallanat, J. House, M.C. Schladweiler, C. Wood, and U. Kodavanti. Peripheral Metabolic Effects of Ozone Exposure in Healthy and Diabetic Rats on Normal or High-Cholesterol Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115427): 1, (2021).

In this study, using male Wistar and Wistar-derived Goto-Kakizaki (GK) rats, which exhibit a non-obese type-2 diabetes phenotype, we investigated whether two key metabolic stressors, type-2 diabetes and a high-cholesterol atherogenic diet, exacerbate ozone-induced metabolic effects. Rats were fed a normal control diet (ND) or high-cholesterol diet (HCD) for 12 weeks starting at 4 week-age and then exposed to filtered air or 1.0-ppm ozone (6h/day) for 1 or 2 days. Metabolic responses were analyzed at the end of each day and after an 18-hour recovery period following the 2-day exposure. This dataset is associated with the following publication: Snow, S., A. Henriquez, A. Fisher, B. Vallanat, J. House, M.C. Schladweiler, C. Wood, and U. Kodavanti. Peripheral Metabolic Effects of Ozone Exposure in Healthy and Diabetic Rats on Normal or High-Cholesterol Diet. TOXICOLOGY AND APPLIED PHARMACOLOGY. Academic Press Incorporated, Orlando, FL, USA, 415(115427): 1, (2021).

Data related to Dye et al pub on ozone and lung pathology. This dataset is associated with the following publication: Dye, J., H. Nguyen, E. Stewart, M. Schladweiler, and C. Miller. Sex differences in impacts of early gestational and peri-adolescent ozone exposure on lung development in rats: Implications for later life disease in humans. AMERICAN JOURNAL OF PATHOLOGY. American Association of Pathologist, 194(9): 1636-1663, (2024).