,Wykaz i analiza wyników sekwencjonowania konstruktów panelu ligaz E3 (Cullin type) przeznaczonych do ekspresji białek rekombinowanych w systemie opartym o bakulowirusy.,

,Wyniki pomiarów stabilności białka TNFR1-DD w różnych warunkach buforujących (buffer screen),

The present invention relates to a method for purifying a cysteine containing recombinantly expressed protein comprising at least 2, preferably 3 or 4 and even more preferably all of the following steps: (a) sulphonation of a lysate from recombinant host cells or lysis of recombinant host cells in the presence of guanidinium chloride followed by a subsequent sulphonation of the cell lysate, (b) treatment with a zwitterionic detergent, preferably after removal of the cell debris, (c) purification of the sulphonated version of the recombinant protein or purification of the sulphonated version of the recombinant protein with subsequent removal of the zwitterionic detergent, with said purification being preferably chromatography, more preferably a Ni-IMAC chromatography with said recombinant protein being a His-tagged recombinant protein, (d) desulphonation of the sulphonated version of the recombinant protein, preferably with a molar excess of DTT, (e) storage in the presence of a molar excess of DTT.

Purified genes encoding a T cell surface antigen from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this antigen are provided. Methods of using said reagents and diagnostic kits are also provided.

Selection (M1) of genotype variants from libraries in a micro-structure comprising feeding a test fluid (102) containing a genotype library in an expressible form and with suitable expression aids into a micro-structure together with a barrier fluid (101) to form separate compartments (109,111) in the test fluid, is new. An assay fluid (103) can be added to the test fluid compartments in (M1). The compartments are carried through the micro-structure to give an expression of the genotype in the phenotype in the compartments, for the phenotypes to be detected. The compartments are selected according to their detected phenotypes.

,The data presents the antimicrobial susceptibility testing results in three separate files: 1) third generation cephalosporin resistant E. coli isolates obtained on cefotaxime supplemented media; 2) extended spectrum beta-lactamase (ESBL) producing E. coli, and 3) ESBL-producing Klebsiella, Enterobacter and Citrobacter species obtained on chromogenic media. The data was generated as part of a research project that evaluated the impact of tylosin supplementation of feedlot cattle on the dynamics of antimicrobial resistant fecal bacteria. The study was a longitudinal design with periodic sampling of fecal samples from individual animals over the entire feeding period. Two publications from the project, one describing the study design in detail, and the other specifically reporting on these data, are linked to the database.,Resources in this dataset:,

A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO 166, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.

A Bacillus licheniformis mutant host cell derived from a parent B. licheniformis host cell, which mutant host cell is mutated in one or more gene(s) encoding one or more secreted polypeptide(s) which is at least 80% identical to one or more of the polypeptides shown in SEQ ID NO 166, wherein the mutant host cell secretes at least 5% less of the one or more secreted polypeptide(s) than the parent host cell, when they are cultivated under comparable conditions.

There is provided an antibody or antibody fragment which specifically binds to a portion of SEQ ID NO:2, wherein said portion consists of between 30 and 100 contiguous amino acids of SEQ ID NO:2, and wherein said portion contains amino acids selected from the group consisting of: (a) amino acid residues 54 to 59 of SEQ ID NO: 2; (b) amino acid residues 129 to 134 of SEQ ID NO: 2; (c) amino acid residues 53 to 58 of SEQ ID NO: 2; (d) amino acid residues 35 to 40 of SEQ ID NO: 2; (e) amino acid residues 33 to 38 of SEQ ID NO: 2; (f) amino acid residues 114 to 119 of SEQ ID NO: 2; (g) amino acid residues 101 to 105 of SEQ ID NO: 2; (h) amino acid residues 126 to 131 of SEQ ID NO: 2; (i) amino acid residues 113 to 118 of SEQ ID NO: 2; and (j) amino acid residues 158 to 162 of SEQ ID NO:2.

,Schemat randomizacji regionów hiperzmiennych (CDR) biblioteki PureSelect2 znajdującej zastosowanie w identyfikacji fragmentów przeciwciał zdolnych do wiązania określonych celów molekularnych.,,